Formula to Calculate the Annealing Temperature of Oligonucleotides for PCR
By jeltsch on Wed, 08/30/2006 - 09:47The thumbrule for calculating the annealing temperature for a PCR primer is
Tm (°C) = 81.5 + 0.41(%GC) - (675/N)
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The thumbrule for calculating the annealing temperature for a PCR primer is
Tm (°C) = 81.5 + 0.41(%GC) - (675/N)
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