Kapa HiFi excels, Phusion works sort-of, Q5 and Platinum SuperFi disappoint

Comparison of PCR polymerases

My last NEBuilder assembly got stuck because I couldn't get two of the PCR reactions to work. So I tested alternatives to the Phusion DNA polymerase, which I have been using exclusively for cloning purposes during the last years...

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1000 times too slow

This is all what was needed to connect the XP machine back to the network!

Helsinki University has outsourced parts of its IT infrastructure to Microsoft. Even if outsourcing is cheaper in the short term than generating equivalent services locally, the net effect is likely negative due to the lost jobs, know how and independence. It doesn’t need a conspiracy to explain this self-destructive behavior, just bad decision criteria, which do not include long term and externalized costs.

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