How to transfer the oligo database from MacVec tor to a web-based blast database

First published March 6, 2007 • Last modified on July 18, 2026 • 1 min read • 103 words
  1. Save all sequences in one flatfile format (NOT MacVector format), e.g. genbank format.2. Copy all sequences to a Linux/UNIX computer and convert them from Mac format to UNIX format: mac2unix *.gb3. Write all sequence fiule names into one file: ls *.gb > oligo.lst4. Convert all sequences into one file of concatenated fasta entries using EMBOSS: seqret -sequence @oligo.lst -osformat fasta. Call the output file “mcbl_oligo_db"5. Put this fasta file into the blast web servers database directory (…/blast/db)6. Format the database: formatdb -i mcbl_oligo_db -p F -o T7. Edit …/blast/blast.html by adding the new database name8. Edit …/blast/blast.rc by adding the new database name