t_coffee still fails on a standard Ubuntu 24.04 LTS install

Thu, 18 Sep 2025 00:00:00 +0000

The bug in t_coffee, reported on https://github.com/cbcrg/tcoffee/issues/27#issuecomment-1355339411, is still an issue after many years. I hardly dare to recommend t_coffee to my students, even though I would like to, because it is otherwise an excellent and very powerful program. Most of them fail to install it on our university’s default Ubuntu distribution (“Cubbli”), which is atm Ubuntu 24.04. I tried it out myself just recently (Ubuntu 24.04 LTS with both the version provided by the default Ubuntu repository via the package manager and the stable and beta versions from https://tcoffee.org/Projects/tcoffee/index.html (stable COFFEE_installer_Version_13.46.0.919e8c6b_linux_x64.tar.gz and beta T-COFFEE_installer_Version_13.46.1.b8b01e06_linux_x64.tar.gz). All of them still complain with –ERROR: MAX_N_PID exceeded. It gets stuck somewhere and takes approximately one minute before it throws the error, even with a simple task that normally takes a few seconds. With a more complex alignment, it can take minutes or hours before the error is thrown. The workaround is to set the environment variable before every run, i.e., you replace t_coffee with a shell script that calls the renamed t_coffee after setting the environment parameter MAX_N_PID_4_TCOFFEE to something big (like /proc/sys/kernel/pid_max). The issue is explained in the Github link above. See also https://github.com/cbcrg/tcoffee/issues/47 . Another workaround is to recompile with a different MAX_N_PID, which is rather straightforward; see also here https://github.com/cbcrg/tcoffee :

Overstretched IT support

Fri, 23 Feb 2024 00:00:00 +0000

The problem: Crash during boot

More than a year ago, a few weeks after receiving my new work computer, it failed to reboot after a system update and got stuck early in the boot process. I soon realised I could still start the computer using “safe mode”. Strangely, nothing seems to be wrong because when I manually exit safe mode at the end of the boot process, the computer works fine. Our IT department has tried to fix the problem many times without success. I even had to work without a computer for about 3 weeks while it was “under repair”. You probably know how much work you can get done without a computer: close to zero.My computer runs Cubbli 20.04.06LTS, an unofficial Ubuntu spin maintained by the University of Helsinki for internal use. Although you can do bioinformatics on a Windows or macOS computer, Linux is hands-down the first choice. Many bioinformatics developers don’t even bother to release their software for Windows. MacOS works mostly fine (since it is also UNIX-compliant OS), but I would have to pay twice the price for the same calculating power.The computer is a Lenovo P350 with an NVIDIA T1000 graphics card, which I use to address two screens. I mainly need the graphics card for 3D modelling, phylogenetics analysis and similar tasks. This graphics card may have caused the trouble. I initially did not want to buy this model, but since there was a shortage of graphic cards at the time, IT convinced me to swap out my original choice against the T1000.

Bug on Michael’s Domain

t_coffee still fails on a standard Ubuntu 24.04 LTS install

Overstretched IT support