https://jeltsch.org/en/tags/calculator/Recent content in Calculator on Michael’s DomainHugoen-usCopyright © 2002 - 2026 Michael Jeltsch.Fri, 24 Jul 2026 00:18:18 +0300https://jeltsch.org/en/restriction_enzyme_calculator/Tue, 29 Apr 2025 00:00:00 +0000https://jeltsch.org/en/restriction_enzyme_calculator/<p>Use our interactive tool to calculate the volume of restriction enzyme required for your digest.</p> <div class="card p-4 shadow-sm mb-4"> <h3 id="enzyme-calculator-heading">Restriction Digest Calculator</h3> <form id="calcForm"> <div class="mb-3"> <label for="masstarget" class="form-label">&micro;g of target DNA:</label> <input type="number" step="any" id="masstarget" class="form-control" required /> </div> <div class="mb-3"> <label for="cutstarget" class="form-label">Amount of cuts in target DNA:</label> <input type="number" step="any" id="cutstarget" class="form-control" required /> </div> <div class="mb-3"> <label for="lengthtarget" class="form-label">Length of target DNA in bp:</label> <input type="number" step="any" id="lengthtarget" class="form-control" required /> </div> <div class="mb-3"> <label for="time" class="form-label">Digestion time in hours:</label> <input type="number" step="any" id="time" class="form-control" required /> </div> <div class="mb-3"> <label for="enzyme" class="form-label">Select restriction enzyme:</label> <select name="enzyme" id="enzyme" class="form-select" required></select> </div> <button type="submit" class="btn btn-primary">Calculate</button> </form> <div id="calcForm-results" class="mt-4" style="display: none;"></div> </div> <script src="https://jeltsch.org/js/calculator.min.0c4b18c664d60ee99f8204d704a18528cb4253d10262904935baf9aa2ce28782.js" integrity="sha256-DEsYxmTWDumfggTXBKGFKMtCU9ECYpBJNbr5qizih4I="></script> <p>Sometimes, you need to know how much restriction enzyme is required to cut a specific amount of a certain plasmid within a given time. Here is the calculation tool you have been looking for! All data that was used to write the code for this algorithm was obtained from New England Biolabs (NEB). The survival of the enzyme in the reaction was extrapolated from the experiments reported by NEB. Just plug in the numbers into the form and hit the submit button!</p>https://jeltsch.org/en/toni_nett_s_rules/Sat, 16 Sep 2017 00:00:00 +0000https://jeltsch.org/en/toni_nett_s_rules/<p>More than 50 years ago <a href="https://de.wikipedia.org/wiki/Toni_Nett" target="_blank" rel="noopener noreferrer nofollow">Toni Nett&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> published his book <em>Der Lauf</em>. In the book, he presented thumb rules to calculate possible personal running records for common distances for middle and long distances. The possible personal bests are calculated based on known performances on the next shorter distance. <a href="https://en.wikipedia.org/wiki/Manfred_Steffny" target="_blank" rel="noopener noreferrer nofollow">Manfred Steffny&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> extended Toni Nett&rsquo;s rules to cover the marathon and the 100 km. He found these rules surprisingly accurate when comparing personal bests of hundreds of runners of different levels from beginner to world champion. I have been writing about these thumb rules previously <a href="https://jeltsch.org/en/tags/running/">here</a> . Now I made a small php script that does the calculation for you.</p>