https://jeltsch.org/en/tags/digest/Recent content in Digest on Michael’s DomainHugoen-usCopyright © 2002 - 2026 Michael Jeltsch.Fri, 24 Jul 2026 00:18:18 +0300https://jeltsch.org/en/restriction_enzyme_calculator/Tue, 29 Apr 2025 00:00:00 +0000https://jeltsch.org/en/restriction_enzyme_calculator/<p>Use our interactive tool to calculate the volume of restriction enzyme required for your digest.</p> <div class="card p-4 shadow-sm mb-4"> <h3 id="enzyme-calculator-heading">Restriction Digest Calculator</h3> <form id="calcForm"> <div class="mb-3"> <label for="masstarget" class="form-label">&micro;g of target DNA:</label> <input type="number" step="any" id="masstarget" class="form-control" required /> </div> <div class="mb-3"> <label for="cutstarget" class="form-label">Amount of cuts in target DNA:</label> <input type="number" step="any" id="cutstarget" class="form-control" required /> </div> <div class="mb-3"> <label for="lengthtarget" class="form-label">Length of target DNA in bp:</label> <input type="number" step="any" id="lengthtarget" class="form-control" required /> </div> <div class="mb-3"> <label for="time" class="form-label">Digestion time in hours:</label> <input type="number" step="any" id="time" class="form-control" required /> </div> <div class="mb-3"> <label for="enzyme" class="form-label">Select restriction enzyme:</label> <select name="enzyme" id="enzyme" class="form-select" required></select> </div> <button type="submit" class="btn btn-primary">Calculate</button> </form> <div id="calcForm-results" class="mt-4" style="display: none;"></div> </div> <script src="https://jeltsch.org/js/calculator.min.0c4b18c664d60ee99f8204d704a18528cb4253d10262904935baf9aa2ce28782.js" integrity="sha256-DEsYxmTWDumfggTXBKGFKMtCU9ECYpBJNbr5qizih4I="></script> <p>Sometimes, you need to know how much restriction enzyme is required to cut a specific amount of a certain plasmid within a given time. Here is the calculation tool you have been looking for! All data that was used to write the code for this algorithm was obtained from New England Biolabs (NEB). The survival of the enzyme in the reaction was extrapolated from the experiments reported by NEB. Just plug in the numbers into the form and hit the submit button!</p>