<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>MacOSX on Michael’s Domain</title><link>https://jeltsch.org/en/tags/macosx/</link><description>Recent content in MacOSX on Michael’s Domain</description><generator>Hugo</generator><language>en-us</language><copyright>Copyright © 2002 - 2026 Michael Jeltsch.</copyright><lastBuildDate>Fri, 24 Jul 2026 00:18:18 +0300</lastBuildDate><atom:link href="https://jeltsch.org/en/tags/macosx/index.xml" rel="self" type="application/rss+xml"/><item><title>Opening old plasmid map files</title><link>https://jeltsch.org/en/gck/</link><pubDate>Sat, 01 Jan 2022 00:00:00 +0000</pubDate><guid>https://jeltsch.org/en/gck/</guid><description>&lt;p&gt;For a new cloning project, we needed to access the plasmid maps of an old construct of mine (pSecTagN2, which was a precursor of 
 &lt;a href="https://doi.org/10.1074/jbc.M511593200" target="_blank" rel="noopener noreferrer nofollow"&gt;pMosaic&amp;nbsp;






 
 
 
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, aka pSecTagI) which I had composed from many different sources in 1999. In 1999, I was working in 
 &lt;a href="https://www2.helsinki.fi/en/researchgroups/translational-cancer-biology" target="_blank" rel="noopener noreferrer nofollow"&gt;Kari Alitalo’s laboratory&amp;nbsp;






 
 
 
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 as a Ph.D. student. We were at the &amp;ldquo;cutting edge&amp;rdquo; of technology because we used a software program called 
 &lt;a href="http://www.textco.com/gene-construction-kit.php" target="_blank" rel="noopener noreferrer nofollow"&gt;Gene Construction Kit&amp;nbsp;






 
 
 
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 (GCK) to keep track of our clonings. Last Wednesday, I spent 4 hours of my working time opening one file created with GCK version 2.5 in 1999.&lt;/p&gt;</description></item></channel></rss>