https://jeltsch.org/en/tags/plasmid-documentation/Recent content in Plasmid Documentation on Michael’s DomainHugoen-usCopyright © 2002 - 2026 Michael Jeltsch.Fri, 24 Jul 2026 00:18:18 +0300https://jeltsch.org/en/emboss_and_gck_for_the_assembly_and_documentation_of_construct_sequences/Thu, 05 Apr 2007 00:00:00 +0000https://jeltsch.org/en/emboss_and_gck_for_the_assembly_and_documentation_of_construct_sequences/<p>I am trying to use EMBOSS for the assembly of vector sequences. Long time ago, I used the CGC seqed program for this purpose and at the moment I use the <a href="http://www.textco.com" target="_blank" rel="noopener noreferrer nofollow">Gene Construction Kit&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> . EMBOSS doesn&rsquo;t have a straight equivalent for seqed and one has to use a bunch of other tools to replace its functionality. Look at this <a href="http://helix.nih.gov/apps/bioinfo/emboss-gcg.html" target="_blank" rel="noopener noreferrer nofollow">comparison between CGC and EMBOSS&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> .</p>