https://jeltsch.org/en/tags/pnas/Recent content in PNAS on Michael’s DomainHugoen-usCopyright © 2002 - 2026 Michael Jeltsch.Fri, 24 Jul 2026 00:18:18 +0300https://jeltsch.org/en/vegf_c_vegfr_2_complex_structure_took_13_years_to_solve/Wed, 03 Feb 2010 00:00:00 +0000https://jeltsch.org/en/vegf_c_vegfr_2_complex_structure_took_13_years_to_solve/<p>Finally our paper was accepted for publication in <a href="http://www.pnas.org/content/early/2010/01/19/0914318107.abstract" target="_blank" rel="noopener noreferrer nofollow">PNAS&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> (downloadable also from <a href="https://jeltsch.org/downloads/LeppanenVeli-Matti_PNAS2010.pdf">here</a> ). I started the project by making the first construct 13 years ago, but it went nowhere for the first 9 years due to insufficient concentration of efforts and a few unlucky choices in the experimental design. I opted for bacterial protein first, but although the refolding worked, it was very inefficient (Ala mutation and a smart trimming of N- and C-terminus. <a href="http://www.med.helsinki.fi/uutiset/2010/2010019_Leppanen.htm" target="_blank" rel="noopener noreferrer nofollow">More…&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> </p>