https://jeltsch.org/en/tags/protein-folding/Recent content in Protein Folding on Michael’s DomainHugoen-usCopyright © 2002 - 2026 Michael Jeltsch.Fri, 24 Jul 2026 00:18:18 +0300https://jeltsch.org/en/ecoli-vegfc/Fri, 01 Jul 2022 00:00:00 +0000https://jeltsch.org/en/ecoli-vegfc/<p>The preprint of our manuscript is online. We show how to produce bioactive mature VEGF-C in the cytoplasm of <em>E. coli</em> bacteria without the need for a folding step. It took us quite a while to get there, and we tried many things that did not work before we found a way how to do it. We describe also the methods that failed. It looks as if VEGF-C has simply too many cysteine residues that all have to pair up in the correct configuration. In the same manuscript, we also report a workable refolding method. Please have a look and give us some feedback: <a href="https://www.researchsquare.com/article/rs-1776636/v1" target="_blank" rel="noopener noreferrer nofollow">https://www.researchsquare.com/article/rs-1776636/v1&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> .</p>