Software on Michael’s Domain

Barrier (a software KVM switch)

Tue, 21 Jan 2025 00:00:00 +0000

Barrier is an incredible software that lets you use the same keyboard and mouse with different computers without the need for hardware. I have two computers on my desk, and I used to have a hardware KVM (keyboard, video, and mouse) switch, which became obsolete when my computers started to output their video via HDMI. Hence, I have two keyboards on my desk, which is not very ergonomic. I knew about software solutions to this dilemma, but I never had the time to implement them, and I also did not want to pay a subscription fee for this luxury. I now started to use Barrier: https://github.com/debauchee/barrier . It’s been working excellently so far (except for the installation and documentation), but I still need to get it to work before logging in to dump my second keyboard.here are the steps that were not well documented:When installed on Ubuntu, Barrier does not automatically create certificates. So you need to do this yourself:Execute in both client and server: /home/username/.local/share/barrier/SSL:openssl req -x509 -nodes -days 365 -subj /CN=Barrier -newkey rsa:4096 -keyout Barrier.pem -out Barrier.pemYou also need to open port 24800 if you are using a firewall. After you get Barrier working, check the correct command (ps aux) from the command line. It is probably something like this:/usr/bin/barrierc -f --no-tray --debug INFO --name computer-name [87.167.231.88]:24800startUse the Startup applications in Ubuntu and paste the above command in. This means you cannot use Barrier to log in because it is loaded only after logging in. But that’s as much as I managed to figure out so far. There is probably a way to make this work pre-login via systemd, but I did not have the time to figure out how.

Ouriginal fails more often than not to detect plagiarism

Thu, 23 May 2024 00:00:00 +0000

Ouriginal , the plagiarism detection service formerly known as Urkund, has been in use at the University of Helsinki for a very long time. Because of Ouriginal’s inability to process some of our students’ Master’s thesis, I spent a little bit of time investigating Ouriginal and its problems. Here’s what I found out. From Swedisch to European to US-AmericanIt is unclear to me which software we are really using since Ouriginal is a merger of the Swedish Urkund and the German PlagScan, and in 2021 Ouriginal was acquired by the American Turnitin . I don’t need any of such software, but our university mandates that theses must be scanned by the software. Since I read and evaluate an increasing number of theses every year, I also increasingly use Ouriginal. Ouriginal supports many document formats, but support is buggy Looking at the history of submissions, I realized that until about mid-May 2024, all submitted PDFs had been dutifully analyzed. But last week, something changed since about half of the submitted documents were not processed. The usual tricks (submitting in another format such as .docx, .odt, .ps, .rtf) did work for some, but not all of the theses. One thesis was especially stubborn, and neither our “experts” nor the Original helpdesk staff had any clue why their software could not process it (which makes me again wonder what software their backend is actually using). Ouriginal is investigating already for a month without getting back to me with answers… Our university receives funding from the Ministry of Education based on graduating students, and we lose thousands of Euros for each student who fails to graduate on time. Fee-liable students also have a strong incentive to graduate on time in order to avoid additional costs. Therefore, every delay in the graduation schedule is unacceptable. Ouriginal’s performance disappoints with a shocking detection rate Since there are dozens of ways how to turn a Word document into a PDF, I tried to isolate the problem by submitting test documents to the Ouriginal service. I used manuscripts that we had published over the last few years. I was shocked when I realized that Ouriginal failed to detect plagiarism in more than half of all cases. Paywalls are not to blame since almost all our publications are freely available. Neither the novelty of the publication nor the quality of the journal made a perceivable difference. However, it seems evident that Ouriginal has more problems with non-English documents. It is very difficult to explain why so many freely available scientific texts from reputable journals ( Scientific Reports , Angiogenesis , Annals of Anatomy ) are not indexed by Ouriginal. Failure to detect plagiarism from Helsinki University’s own thesis repository Original does not even index our own university’s freely available PhD theses ( https://ethesis.helsinki.fi ). None of the PhD thesis written in my own lab was recognized. I also checked my own PhD thesis , and it was the only one that was recognized (Ouriginal found it on docslib.org , not our own university’s official thesis repository). Does it make any sense to pay for such an imperfect service? I don’t think so. The omission of freely available texts from important journals seems inexcusable since a simple Google search does a better job. I did Google searches for sentences from the unrecognized texts, and in most cases, Google identified the original source without problems. A Python script to split texts into sentences for individual Google searches and to aggregate the results can be written by any programmer in an afternoon. Why would you maintain your own database if Google does a better job than you can? Plagiarism to the following published manuscripts/theses/proceedings was NOT detected:*

Ḿanuscript reviewing by annotating PDFs

Fri, 28 Jun 2019 00:00:00 +0000

Manuscripts for scientific peer-review are delivered as PDF files. Thus it appears most natural to comment the PDF file itself instead of submitting the comments as a separate text (file). However, many submission systems do not allow to submit comments in form of an annotated PDF file.In addition, there is no easy-to-use free/Open Source PDF editor for Linux (my platform of choice). Yes, there is PDFEdit ( http://pdfedit.cz/en/index.html) , but it requires substantial learning and its last release dates back to 2012. There are free online PDF editors (e.g. https://www.pdfescape.com/) , but sometimes I need a local tool. Although not Open Source and not free, the tool of my choice has been PDF Studio Pro . It has all the features I need (actually much more than I need), it is truly cross-platform (Windows, Mac, Linux), easy to use and very affordable for what it offers ($129 single permanent license). Unfortunately, Quoppa software - the maker of PDF Studio - does not offer academic discounts.PDF Studio ViewerA while ago, the makers of PDF Studio started to offer a free version called PDF Studio Viewer . This free version has gotten more useful over time. Since last year, it also supports PDF annotation, which is very important for my work, mostly when I am peer-reviewing scientific manuscripts. For many academic users, the PDF Studio Viewer might be fully sufficient.Libre Office and InkscapeOf course Libre Office Draw and Inkscape can edit PDFs, but they are not specialized for annotating. If you need to do extensive annotations, the process becomes soon very painful. In addition, Inkscape editing can be destructive (i.e. when you modify text), but I have used it e.g. to fill out forms.Apart from the lack of dedicated annotation and reviewing tools (“markups” like “replace text”, “crossout text”, “delete text”, “insert text” or callouts), Libre Office Draw is actually a quite capable PDF editor, but fails still sometimes to correctly open very complex PDF documents (I have had problems with background images/patterns). Strangely, the only reviewing tool (comments) are not exported by default. You need to check the “Export comments” box when you export your edited PDF file as PDF (the “Save” operation creates an ODG file, which you probably don’t want).PDFsamIf you do not have the need to annotate, then you might get away with the Open Source tool PDFsam . Unlike PDFEdit, PDFsam is available from the Ubuntu repositories. PDFsam also has two non-free versions ( PDFsam Enhanced and PDFsam Visual ). PDFsam Basic (the Open Source version) is just a simple GUI on top of some command line utilities, whereas the PDF Studio Viewer offers a true visual editing experience. I canot talk about the non-free versions of PDFsam as they are not available as free trials. In fact, all of the operations of PDFsam can be achieved via the command line (see here for my blog post about how to perform common PDF editing tasks using mostly the command line tool pdftk ).Reducing file sizePDFsam Basic and PDF Studio Viewer do not offer any functionality to reduce file size. The LibreOffice Draw PDF export dialog let’s you reduce image resolution and JPEG compression, which you can use to reduce file size. But at the Open Source front, the only capable tools to reduce PDF size seem to be command line tools. I use ghostscript for this task, but the command is not easy to remember:gs -sDEVICE=pdfwrite -dCompatibilityLevel=1.4 -dPDFSETTINGS=/screen -dNOPAUSE -dQUIET -dBATCH -sOutputFile=output.pdf input.pdfWith PDF Studio Pro, you obviously do not need to remember the different keywords for the different output quality option (screen, ebook, printer, prepress) as you just choose from the drop down menu between the available options. If you are looking for an Open Source graphical wrapper for ghostscript, perhaps try workerPdf . Signing documents and adding signaturesPDF Studio Viewer let’s you sign documents if you have a DocuSign subscription . However, I am mostly concerned about being able to prove myself that I created a certain document (“self-signed signature”) and not that others are able to verify my authorship. For this scenario you are out of luck with PDF Studio Viewer. And apparently, PDFSam does not offer any possibility for signing (neither self-signing nor 3rd party signing). However, Libre Office Draw allows documents signing! I have been looking for an affordable solution (not self-signed, but trusted by other PDF readers) to sign PDF documents, but there seems to be no appropriate solution if you need to sign only rarely. The basic plan by DocuSign ($10/month) appears too expensive when signing only one document per month.Take-home messageIf you want to stay with free or Open Source solutions, you probably need to combine several tools in order to cover all typical PDF editing tasks without pain: PDFsam Basic, LibreOffice Draw, PDF Studio Viewer and ghostscript. But if you edit PDFs often (as I do), buying PDF Studio Pro is clearly the way to go.

SnapGene and partial restriction digests revisited

Thu, 23 Aug 2018 00:00:00 +0000

Snapgene is a software for the wet lab molecular biologist, who does lots of cloning work (construct design and annotation). Since I last wrote about the SnapGene software ( https://www.snapgene.com/ ), many good things have happened:

SnapGene - Simply the best DNA manipulation software

Fri, 01 Jan 2016 00:00:00 +0000

Our lab has been using different software packages to plan, document and visualize DNA constructs. Among those that we liked a lot for a long time were Textco’s GeneConstructionKit (GCK) and Clone Manager (Professional) . The latter runs unfortunately only under Windows. However, since several of our computers run Ubuntu Linux , we did run GCK versions 2.5 and 3 using WINE (a compatibility layer that allows us to run native Windows programs under Linux). However, with the upgrade to version 4, GCK became unusably slow under WINE and we were looking for a replacement. We contacted the developers of GCK, but they apparently were either not willing or able to help us. I suppose that the codebase of GCK is probably more than 20 years old and for that reason nobody dares to touch it. Just around that time, SnapGene was released and it fulfilled almost all of our requirements:

Software development projects for Molecular Biology

Thu, 05 Apr 2007 00:00:00 +0000

GCK2.5-related

GCK2.5 debug under wine** There are still some bugs that make using gck2.5 sometimes a pain under wine. Especially the inability to annotate regions, to search for a sequence and to open a new file.
GCK2.5 export** GCK2.5 is not able to export in embl format with the regions converted into features. It can, however, export comments to text file and plain sequence to a text file. It should be trivial to write a perl script that takes these two files and converts them into one embl file, EMBOSS cirdna/lindna or pDRAW32 file.
GCK2.5/wine desktop integration** When clicking on files that are associated with Windows programs (using wine), the Linux file manager (e.g. Konqueror) passes the file as an argument to the associated Windows application and the file is opened under wine. However GCK2.5 refuses to accept the file as an argument. When clicking on a .gcc file, GCK2.5 starts up, but opens an empty window and I have to open the .gcc file from within GCK2.5. Unnecessary clicking, especially when I need to navigate over several folder hierachies. When GCK2.5 is running natively under Windows, is it possible to start GCK2.5 with a construct file as a command line argument? I should check that out.

pDRAW32-related