https://jeltsch.org/en/tags/stripping/Recent content in Stripping on Michael’s DomainHugoen-usCopyright © 2002 - 2026 Michael Jeltsch.Fri, 24 Jul 2026 00:18:18 +0300https://jeltsch.org/en/stripping/Fri, 01 Nov 2013 00:00:00 +0000https://jeltsch.org/en/stripping/<p>Reprobing membranes with with a different antibody is a very common task in the lab. Various protocols exist to strip membranes and the classic method is the one that uses SDS, β-mercaptoethanol and heating. I used to do it that way, but it&rsquo;s a smelly business, because β-mercaptoethanol smells like rotten eggs. Then suddenly everybody in the lab started to use the <a href="http://www.millipore.com/catalogue/item/2504" target="_blank" rel="noopener noreferrer nofollow">Re-Blot Plus&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> Solution from Millipore and so did I. Until I realized by chicking the <a href="http://www.millipore.com/msds.nsf/a73664f9f981af8c852569b9005b4eee/85256f0a005296f2852575d6006fc2b2/$FILE/00000123MSDS.pdf" target="_blank" rel="noopener noreferrer nofollow">Material Safety Data Sheet&nbsp; <svg class="svg-inline--fa fas fa-up-right-from-square fa-2xs" fill="currentColor" aria-hidden="true" role="img" viewBox="0 0 512 512" overflow="visible"><use href="#fas-up-right-from-square"></use></svg></a> that they sell cheap chemicals for a premium price. Now I make the stripping buffer myself. My 10x solution has the following composition:</p>