The Jeltsch Laboratory on Michael’s Domain

Bioactive VEGF-C from E. coli without in-vitro folding!

Fri, 28 Oct 2022 00:00:00 +0000

Our article Bioactive VEGF-C from E. coli has been published in Scientific Reports. Read here: https://doi.org/10.1038/s41598-022-22960-0.As a matter of fact, I did the first experiments expressing VEGF-C in E. coli in 1999, but never got active protein. In the following 15+ years, we exclusively used eukaryotic cells (yeast, S2/Sf9/Hi5 insect cells, CHO) to produce VEGF-C. We reactivated the “VEGF-C in E. coli” project a few years back and we finally reached our goal last year. However, it was much more work than we originally anticipated. In the beginning, all attempts went South, and to rescue the project, we developed an in-vitro folding protocol. It was perhaps more luck than ability that we stumbled upon a combination of solubility tag and redox-modified E. coli strain that can pull off the trick to produce directly bioactive VEGF-C without the need for an in-vitro folding step. We decided to include also our unsuccessful attempts (CyDisCo and periplasmic expression) in the results section to avoid the file drawer effect.

Jeltsch Lab participates in Helsinki Running Day 2018

Wed, 23 May 2018 00:00:00 +0000

Our lab participated in the Helsinki Running Day with great results! The “Biomedicum Runners” marathon relay team: Sawan Kumar Jha, Timo Lehti, Khushbu Rauniyar, Rustem Kasymov. Individual performances by Zalina Magomedova, Michael Jeltsch (both marathon) and Alish GM (5k). Missing from the picture is Alisha GM.

Acatiimi article about our lab

Mon, 27 Nov 2017 00:00:00 +0000

However - unlike in the previous story - this time there is a group image, showing who does the real work!

Featured again…

Thu, 26 Oct 2017 00:00:00 +0000

Recently, our research seems to get lots of public interest: https://50tieteentekijaa.fi/tieteentekija/michael-jeltsch/ . And there is more to come… It would be nice if our government would show a similar interest in science and education. And it would be even better if they had a reasonable plan how to secure the future of science and research in Finland. The government spending for some research areas (in other words the investment into Finland’s future) has been shrinking so dramatically, that even the tabloids have picked up the topic: http://www.iltalehti.fi/kotimaa/201710122200451078_u0.shtml

Our key project featured

Wed, 25 Oct 2017 00:00:00 +0000

Our key project was featured in Tietyssti.fi . Katri Pajusola wrote the story and (at least from what I understand from the Finnish text) it seems to be a quite accurate description of what we try to do: http://www.aka.fi/fi/tietysti/luonto-ja-ymparisto/nyt-pinnalla1/tautien-hoitoon-laakitysta-ei-ainoastaan-diagnostiikkaa/