<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Thermo Fisher Scientific on Michael’s Domain</title><link>https://jeltsch.org/en/tags/thermo-fisher-scientific/</link><description>Recent content in Thermo Fisher Scientific on Michael’s Domain</description><generator>Hugo</generator><language>en-us</language><copyright>Copyright © 2002 - 2026 Michael Jeltsch.</copyright><lastBuildDate>Fri, 24 Jul 2026 00:18:18 +0300</lastBuildDate><atom:link href="https://jeltsch.org/en/tags/thermo-fisher-scientific/index.xml" rel="self" type="application/rss+xml"/><item><title>The case against FastDigest®</title><link>https://jeltsch.org/en/the_case_against_fastdigest/</link><pubDate>Wed, 25 Jun 2014 00:00:00 +0000</pubDate><guid>https://jeltsch.org/en/the_case_against_fastdigest/</guid><description>&lt;p&gt;&lt;em&gt;&lt;strong&gt;Restriction enzymes continue to be important&lt;/strong&gt;&lt;/em&gt;&lt;/p&gt;
&lt;p&gt;In 2006, the innovative Lithuanian biotech company 
 &lt;a href="http://en.wikipedia.org/wiki/Fermentas" target="_blank" rel="noopener noreferrer nofollow"&gt;Fermentas&amp;nbsp;






 
 
 
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 launched a new product line for molecular biology researchers: the FastDigest restriction enzymes. Together with the polymerase chain reaction (PCR), restriction enzymes (REs) are arguably the most important tools in molecular biology. They made recombinant DNA technology possible in the early 1970s. Until every lab can afford a reliable 
 &lt;a href="http://cambriangenomics.com" target="_blank" rel="noopener noreferrer nofollow"&gt;DNA laser printer&amp;nbsp;






 
 
 
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 (which is probably still a decade away), restriction enzymes are the tools of the trade. Novel cloning techniques (like 
 &lt;a href="http://bioinfo.clontech.com/infusion/" target="_blank" rel="noopener noreferrer nofollow"&gt;In-Fusion&amp;nbsp;






 
 
 
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, 
 &lt;a href="https://www.neb.com/applications/cloning-and-synthetic-biology/gibson-assembly-cloning" target="_blank" rel="noopener noreferrer nofollow"&gt;Gibson Assembly&amp;nbsp;






 
 
 
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 or 
 &lt;a href="http://nar.oxfordjournals.org/content/early/2012/01/11/nar.gkr1288.full" target="_blank" rel="noopener noreferrer nofollow"&gt;SLICE&amp;nbsp;






 
 
 
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) have their place, but still lack the robustness of traditional restriction enzyme cloning.&lt;/p&gt;</description></item></channel></rss>