GCK

After working with GCK2.5 under wine I have identified more bugs that make the program quit unexpectedly. However, now I have not been using cross-over office (as its evaluation period has expired and of course my boss wouldn't spend a cent for Linux programs). So I am using the default installation of wine that comes with Suse 9. I copied over the fake windows directory from the former .cxoffice directory. The following bugs do exist (they always appear when a window opens that requires you to enter alphanumeric values via the keyboard):

Obviously you can import sequences into GCK2.5 directly from Genbank. But usually you are working with a web tool like SRS and end up having your sequences displayed in your browser. From SRS version 7 the import goes as follows: From your fancy display, click SAVE. From the pull-down menu "Use view" select * Complete Entries *. Then "Output to" FILE (TEXT). Check under "Save as type" SAVE TABEL AS ASCii TABLE/TEXT WITH. Click SAVE and save it somewhere with the file extension .ebl. Then from within GCK2.5 select IMPORT and select the file. Ready.

I am trying to use EMBOSS for the assembly of vector sequences. Long time ago, I used the CGC seqed program for this purpose and at the moment I use the Gene Construction Kit. EMBOSS doesn't have a straight equivalent for seqed and one has to use a bunch of other tools to replace its functionality. Look at this comparison between CGC and EMBOSS.