Linux

Installing mplayer on kubuntu

  1. edit /etc/apt/sources.list and uncomment the lines for universe (remove the "" in front of the lines)
  2. add a line similar to universe see the example: http://fi.archive.ubuntu.com/ubuntu breezy multiverse
  3. download any extra codecs: wget http://www2.mplayerhq.hu/MPlayer/releases/codecs/all-20050412.tar.bz2
  4. unpack the codecs: tar -xvjf all-20050412.tar.bz2
  5. as sudo:

How to transfer the oligo database from MacVec tor to a web-based blast database

1. Save all sequences in one flatfile format (NOT MacVector format), e.g. genbank format.
2. Copy all sequences to a Linux/UNIX computer and convert them from Mac format to UNIX format: mac2unix *.gb
3. Write all sequence fiule names into one file: ls *.gb > oligo.lst
4. Convert all sequences into one file of concatenated fasta entries using EMBOSS: seqret -sequence @oligo.lst -osformat fasta. Call the output file "mcbl_oligo_db"
5. Put this fasta file into the blast web servers database directory (.../blast/db)

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