DNA base ambiguity codes
By jeltsch on Wed, 08/30/2006 - 10:38code unresolved bases M AC R AG W AT S CG Y CT K GT V ACG H ACT D AGT B CGT N ACGT
English
code unresolved bases M AC R AG W AT S CG Y CT K GT V ACG H ACT D AGT B CGT N ACGT
The thumbrule for calculating the annealing temperature for a PCR primer is
Tm (°C) = 81.5 + 0.41(%GC) - (675/N)
of things I always forget...