How much PCR product can you get?
By jeltsch on Thu, 03/10/2016 - 09:44
How much PCR product does one get from a typical PCR reaction? 50-120 ng/µl seems to be a typical result, but it very much depends on your PCR conditions.
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PCR
Formula to Calculate the Annealing Temperature of Oligonucleotides for PCR
By jeltsch on Wed, 08/30/2006 - 09:47The thumbrule for calculating the annealing temperature for a PCR primer is
Tm (°C) = 81.5 + 0.41(%GC) - (675/N)
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