Äkta Explorer FPLC core facility

Äkta Explorer FPLC Maschine

Nowadays I spend much of my time at work on the purification of proteins which regulate angiogenesis and lymphangiogenesis. Therefore, I am taking care of the necessary machinery that the Molecular Cancer Biology Research Program owns: the fast protein liquid chromatography (FPLC) machine.

Prof. Jorma Keski-Oja acquired about 15 years ago one of the first (and in 2010 discontinued) Äkta Explorer machines from Swedish producer Pharmacia (who merged in 1997 with Amersham to become Amersham Pharmacia Biotech, who was in turn bought by GE Healthcare in 2004). In the year 2000 the equipment moved from the Haartman Institute into the Biomedicum Helsinki. While I have maintained web pages for this piece of research infrastructure for the last five years (including online reservation and data backup), they were only available from inside the Helsinki University network.

Now, I managed to have web pages about the Äkta Explorer FPLC core facility added to the web site of the Medical Faculty. At the moment, we are adding the WAVE cell culture system to our facility to be able to produce large amounts of cells/cell culture supernatant (up to 25 liters of bacterial, insect or mammalian cell culture) for protein production.