Liste unser meistbenutzten Oligos für DNS-Sequenzierung (interne MCBL-Nummern)

Table: 
TargetOrientationSequenceNumber
3'AOX1 (Hefe-Expressionsvektor)rev5'-GCAAATGGCATTCTGACATCC-3'16094
5'AOX1 (Hefe-Expressionsvektor)fwd5'-GACTGGTTCCAATTGACAAGC-3'16093
a-Factor (Hefe-Expressionsvektor)fwd5'-ACTACTATTGCCAGCATTGCTGC-3'6014
Ac5fwd5'-ACACAAAGCCGCTCCATCAG-3'18939
aMHC Promoterfwd5'-CAGGGAAGTGGTGGTGTAGG-3'8740
aMHC Promoterfwd5'-TCCTCACCCCCTGGCTTGTC-3'3974
BGH pAfwd5'-GATAATAGCAGGCATGCTGG-3'15492
CD33SPfwd5'-GGACTAGTCCCAAGCTGCTTCCTCAGACATGCC-3'18891
CMV Promoterfwd5'-AGTAACGCCAATAGGGACTT-3'16635
CMV Promoterfwd5'-GCTGCTTCGCGATGTACGG-3'15454
CMV Promoterfwd5'-TACTTGGCAGTACATCTACGTATTAGTCATCGC-3'18782
CMV Promoterrev5'-CTGGCTTAACTATGCGGCATC-3'4083
CMV Promoterrev5'-GCTATGAACTAATGACCCCG-3'15520
EBV rev (pREP, Nichtstandartprimer)rev5'-AATAGCATCACAAATTTCAC-3'8435
EBV rev (pREP)rev5'-GTGGTTTGTCCAAACTCATC-3'8725
f1 ori?5'-GCTGATTTAACAAAAATTTAACGCG-3'17733
f1 orirev5'-GTCACGCTGCGCGTAACC-3'12077
GL Primer 2 (pGL3)?5'-CTAGCAAAATAGGCTGTCCC-3'15951
humanes Wachstumshormon polyAfwd5'-TTGCTTGGCACTGTCCTCTC-3'4084
humanes Wachstumshormon polyArev5'-AAATCAGAAGGACAGGGAAGG-3'3852
humanes Wachstumshormon polyArev5'-TAGGACAAGGCTGGTGGGC-3'8739
IgK SPfwd5'-CCATGGAGACAGACACAC-3'11861
IgK SPfwd5'-GGCTCTCTTCCATGGAGACAGACACAC-3'12416
K14 polyAfwd5'-TTTCATGTGTGTCCATTCATTCACC-3'9737
K14 polyArev5'-AATGACCAGAGAGAGGCGAG-3'3862, 17571
K14 polyArev5'-CACCCCCACTCCTAACAATGACC-3'9738
K14 Promoterfwd5'-AGCCAACAGCAACATGCCTCC-3'9741
K14 Promoterfwd5'-GAAAGCCCAAAACACTCCAAAC-3'3861, 17570
K14 Promoterrev5'-TTCCTCTGTGCCCCACCACC-3'9740
lacZfwd5'-GTCAGACATGTATACCC-3'15837
lacZrev5'-CCATTTGCTGTCCACC-3'15836
M13/pUC universeller Forwärtsprimer (-20)fwd5'-GTAAAACGACGGCCAGT-3'none
M13/pUC universeller Rückwärtsprimerrev5'-CACACAGGAAACAGCTATGACCAT-3'none
MTfwd5'-CATCTCAGTGCAACTAAA-3'13455
pCDNA3/BGH pArev5'-TAGAAGGCACAGTCGAGG-3'12057, 17921
pETfwd5'-ATGCGTCCGGCGTAGA-3'17834
pETrev5'-ATCCGGATATAGTTCC-3'11853
pFastBac1rev5'-GGAGGTTTTTTAAAGCAAGT-3'61192 (kein MCBL-Primer)
pIgPLUS SEQ 3'rev5'-ATGTGTGAGGTTTGTCACAAG-3'13456, 17848
pIgPLUS SEQ 5'fwd5'-AAGCAGAGCTCTCTGGCTA-3'15136
Polyhedrin Promoter (Baculovirusvektoren)fwd5'-TTAAAATGATAACCATCTCGC-3'61191 (kein MCBL-Primer)
pREPfwd5'-GCTCGATACAATAAACGCC-3'8724
pREP, Nichtstandartprimerfwd5'-CGTAACGTCTCTATTAACAT-3'8434
pUC8?5'-GTGCTGCAAGGCGATTAAGTTGGG-3'17735
RIP (Ratten Insulin-Promoter)fwd5'-TAATGGGACAAACAGCAAAG-3'3495
RV Primer 3 (pGL3)?5'-CTTTATGTTTTTGGCGTCTTCCA-3'15950
Sp6fwd5'-ATTTAGGTGACACTATAG-3'keine
SV40 polyAfwd5'-TATTTATGCAGAGGCCGAGG-3'12186
SV40 polyArev5'-ACAGACTGTGAGGACTGAGG-3'3496
T3fwd5'-AATTAACCCTCACTAAAGGGA-3'keine
T7 promoter (short primer)fwd5'-TAATACGACTCACTATAGGG-3'17471
T7 Terminatorrev5'-CGTTTAGAGGCCCCAAGG-3'12770
TK pA?5'-GCTCGCAGCCAACGTCGG-3'12078