Practical Course: Purification and Characterization of Recombinant Proteins (DPBM-135)

Teaching material, results, etc. for the DPBM course "Purification and Characterization of Recombinant Proteins" (https://courses.helsinki.fi/en/DPBM-135/120171139):

• FPLC (Fast Protein Liquid Chrmatography)

  Until I add the new data, please refer to this documentation: https://jeltsch.org/FPLC-course

  Protocol (draft version)

  • Protein Purification with the Äkta Avant (PDF)
  • Protein Purification with the Äkta Avant (editable, Scribus file format)

  Software

  • Bio-Rad's ChromLab software for FPLC control and analysis (you need to create an account to get to the download)

  Results

  • Annotated, representative FPLC results of group 2 & 5 (more to come...)
  • Enhanced Windows Metafiles of the chromatograms:
    Group 1, PAS
    Group 2, SEC
    Group 2, PAS
    Group 2, SEC
    Group 5, SEC
  • Scans of the Coomassie-stained SDS-PAGE gels:
    Group 2, reducing SDS-PAGE
    Group 2, non-reducing SDS-PAGE
    Group 3, reducing SDS-PAGE
    Group 3, non-reducing SDS-PAGE

   

• ITC (Isothermal titration calorimetry)

  Some Very Brief Introduction

  • The Working Principle of Isothermal Titration Calorimetry: https://www.youtube.com/watch/?v=o_IpWcWKNXI
  • Experimental Techniques Explained: Isothermal Titration Calorimetry: https://antisensescienceblog.wordpress.com/2013/11/15/experimental-techn...
  • A nice lecture series by Lars Wadsö (Lund University), that was unfortunately never completed: https://www.calmetrix.com/lectures-calorimetry

  Device Documentation

  • PEAQ-ITC Getting Started Guide
  • PEAQ-ITC User Manual (English)
  • PEAQ-ITC Operating Instructions (English)
  • PEAQ-ITC Analysis Software User Manual (English)

  Documentation Videos

  • Cleaning the instrument
  • Loading the instrument

  Software

  • Setup MicroCal PEAQ-ITC Analysis Software
  • Setup MicroCal PEAQ-ITC Control Software