Wenn auch alles anders aussieht, hat sich doch nicht alles verändert...
Liste unser meistbenutzten Oligos für DNS-Sequenzierung (interne MCBL-Nummern)
Von jeltsch am Sa, 01/21/2012 - 20:38
Table:
| Target | Orientation | Sequence | Number |
|---|---|---|---|
| 3'AOX1 (Hefe-Expressionsvektor) | rev | 5'-GCAAATGGCATTCTGACATCC-3' | 16094 |
| 5'AOX1 (Hefe-Expressionsvektor) | fwd | 5'-GACTGGTTCCAATTGACAAGC-3' | 16093 |
| a-Factor (Hefe-Expressionsvektor) | fwd | 5'-ACTACTATTGCCAGCATTGCTGC-3' | 6014 |
| Ac5 | fwd | 5'-ACACAAAGCCGCTCCATCAG-3' | 18939 |
| aMHC Promoter | fwd | 5'-CAGGGAAGTGGTGGTGTAGG-3' | 8740 |
| aMHC Promoter | fwd | 5'-TCCTCACCCCCTGGCTTGTC-3' | 3974 |
| BGH pA | fwd | 5'-GATAATAGCAGGCATGCTGG-3' | 15492 |
| CD33SP | fwd | 5'-GGACTAGTCCCAAGCTGCTTCCTCAGACATGCC-3' | 18891 |
| CMV Promoter | fwd | 5'-AGTAACGCCAATAGGGACTT-3' | 16635 |
| CMV Promoter | fwd | 5'-GCTGCTTCGCGATGTACGG-3' | 15454 |
| CMV Promoter | fwd | 5'-TACTTGGCAGTACATCTACGTATTAGTCATCGC-3' | 18782 |
| CMV Promoter | rev | 5'-CTGGCTTAACTATGCGGCATC-3' | 4083 |
| CMV Promoter | rev | 5'-GCTATGAACTAATGACCCCG-3' | 15520 |
| EBV rev (pREP, Nichtstandartprimer) | rev | 5'-AATAGCATCACAAATTTCAC-3' | 8435 |
| EBV rev (pREP) | rev | 5'-GTGGTTTGTCCAAACTCATC-3' | 8725 |
| f1 ori | ? | 5'-GCTGATTTAACAAAAATTTAACGCG-3' | 17733 |
| f1 ori | rev | 5'-GTCACGCTGCGCGTAACC-3' | 12077 |
| GL Primer 2 (pGL3) | ? | 5'-CTAGCAAAATAGGCTGTCCC-3' | 15951 |
| humanes Wachstumshormon polyA | fwd | 5'-TTGCTTGGCACTGTCCTCTC-3' | 4084 |
| humanes Wachstumshormon polyA | rev | 5'-AAATCAGAAGGACAGGGAAGG-3' | 3852 |
| humanes Wachstumshormon polyA | rev | 5'-TAGGACAAGGCTGGTGGGC-3' | 8739 |
| IgK SP | fwd | 5'-CCATGGAGACAGACACAC-3' | 11861 |
| IgK SP | fwd | 5'-GGCTCTCTTCCATGGAGACAGACACAC-3' | 12416 |
| K14 polyA | fwd | 5'-TTTCATGTGTGTCCATTCATTCACC-3' | 9737 |
| K14 polyA | rev | 5'-AATGACCAGAGAGAGGCGAG-3' | 3862, 17571 |
| K14 polyA | rev | 5'-CACCCCCACTCCTAACAATGACC-3' | 9738 |
| K14 Promoter | fwd | 5'-AGCCAACAGCAACATGCCTCC-3' | 9741 |
| K14 Promoter | fwd | 5'-GAAAGCCCAAAACACTCCAAAC-3' | 3861, 17570 |
| K14 Promoter | rev | 5'-TTCCTCTGTGCCCCACCACC-3' | 9740 |
| lacZ | fwd | 5'-GTCAGACATGTATACCC-3' | 15837 |
| lacZ | rev | 5'-CCATTTGCTGTCCACC-3' | 15836 |
| M13/pUC universeller Forwärtsprimer (-20) | fwd | 5'-GTAAAACGACGGCCAGT-3' | none |
| M13/pUC universeller Rückwärtsprimer | rev | 5'-CACACAGGAAACAGCTATGACCAT-3' | none |
| MT | fwd | 5'-CATCTCAGTGCAACTAAA-3' | 13455 |
| pCDNA3/BGH pA | rev | 5'-TAGAAGGCACAGTCGAGG-3' | 12057, 17921 |
| pET | fwd | 5'-ATGCGTCCGGCGTAGA-3' | 17834 |
| pET | rev | 5'-ATCCGGATATAGTTCC-3' | 11853 |
| pFastBac1 | rev | 5'-GGAGGTTTTTTAAAGCAAGT-3' | 61192 (kein MCBL-Primer) |
| pIgPLUS SEQ 3' | rev | 5'-ATGTGTGAGGTTTGTCACAAG-3' | 13456, 17848 |
| pIgPLUS SEQ 5' | fwd | 5'-AAGCAGAGCTCTCTGGCTA-3' | 15136 |
| Polyhedrin Promoter (Baculovirusvektoren) | fwd | 5'-TTAAAATGATAACCATCTCGC-3' | 61191 (kein MCBL-Primer) |
| pREP | fwd | 5'-GCTCGATACAATAAACGCC-3' | 8724 |
| pREP, Nichtstandartprimer | fwd | 5'-CGTAACGTCTCTATTAACAT-3' | 8434 |
| pUC8 | ? | 5'-GTGCTGCAAGGCGATTAAGTTGGG-3' | 17735 |
| RIP (Ratten Insulin-Promoter) | fwd | 5'-TAATGGGACAAACAGCAAAG-3' | 3495 |
| RV Primer 3 (pGL3) | ? | 5'-CTTTATGTTTTTGGCGTCTTCCA-3' | 15950 |
| Sp6 | fwd | 5'-ATTTAGGTGACACTATAG-3' | keine |
| SV40 polyA | fwd | 5'-TATTTATGCAGAGGCCGAGG-3' | 12186 |
| SV40 polyA | rev | 5'-ACAGACTGTGAGGACTGAGG-3' | 3496 |
| T3 | fwd | 5'-AATTAACCCTCACTAAAGGGA-3' | keine |
| T7 promoter (short primer) | fwd | 5'-TAATACGACTCACTATAGGG-3' | 17471 |
| T7 Terminator | rev | 5'-CGTTTAGAGGCCCCAAGG-3' | 12770 |
| TK pA | ? | 5'-GCTCGCAGCCAACGTCGG-3' | 12078 |