Practical Molecular Biology and Genetic Engineering

Collection of the presentation slides for the DPBM course Practical Molecular Biology and Genetic Engineering. There are files in (at least) two different formats for each lecture: PDF and ODP (Open Document Presentation). The ODP file is editable using LibreOffice software. If you want to open it with Microsoft Office, you need to convert it first using either LibreOffice or some online conversion tool (like cloudconvert). All material is available under the creative commons license. I might add improved versions of the lecture slides later based on participants' feedback. The slide about restriction enzymes (REs) and how to calculate the necessary RE amounts to digest a certain amount of DNA is in the file of Lecture 1.

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PDF icon 2014-09-08 - Lecture 1: The Basics (PDF)
Tiedosto 2014-09-08 - Lecture 1: The Basics (ODP)
PDF icon 2014-09-12 - Lecture 2: Expression Systems (PDF)
Tiedosto 2014-09-12 - Lecture 2: Expression Systems (ODP)
PDF icon 2014-09-19 - Lecture 3: Measuring Proteins (PDF)
Tiedosto 2014-09-19 - Lecture 3: Measuring Proteins (ODP)
PDF icon 2014-09-24 - Lecture 4: Protein Tagging (PDF)
Tiedosto 2014-09-24 - Lecture 4: Protein Tagging (ODP)
PDF icon 2014-10-17 - Lecture 5: "New cloning" methods (PDF)
Tiedosto 2014-10-17 - Lecture 5: "New cloning" methods (ODP)
Package icon 2014-10-17 - Lecture 5: Further reading (ZIPped collection of PDF articles about the "new" "cloning" methods)
PDF icon 2014-10-24 - Lecture 6: Mutagenesis and in-vitro evolution (PDF)
Tiedosto 2014-10-24 - Lecture 6: Mutagenesis and in-vitro evolution (ODP)
Package icon 2014-10-24 - Lecture 6: Further reading (ZIPped collection of PDF articles about mutagenesis and in-vitro evolution methods)
PDF icon 2014-10-31 - Lecture 7: Software for wetlab scientists (PDF)
Tiedosto 2014-10-31 - Lecture 7: Software for wetlab scientists (ODP)
PDF icon 2014-11-07 - Lecture 8: Sequence sources and vector anatomy (PDF)
Tiedosto 2014-11-07 - Lecture 8: Sequence sources and vector anatomy (ODP)
Package icon 2014-11-07 - Lecture 8: Further reading (ZIPped collection of articles about vector design/choice and high thruput cloning)
PDF icon 2014-11-14 - Lecture 9: The Ins and Outs of Knock-Ins and Knock-Outs (PDF)
Tiedosto 2014-11-14 - Lecture 9: The Ins and Outs of Knock-Ins and Knock-Outs (ODP)
PDF icon 2014-11-28 - Lecture 10: Recombinant Viruses (PDF)
Tiedosto 2014-11-28 - Lecture 10: Recombinant Viruses (ODP)