Practical Molecular Biology and Genetic Engineering

Collection of the presentation slides for the DPBM course Practical Molecular Biology and Genetic Engineering. There are files in (at least) two different formats for each lecture: PDF and ODP (Open Document Presentation). The ODP file is editable using LibreOffice software. If you want to open it with Microsoft Office, you need to convert it first using either LibreOffice or some online conversion tool (like cloudconvert). All material is available under the creative commons license. I might add improved versions of the lecture slides later based on participants' feedback. The slide about restriction enzymes (REs) and how to calculate the necessary RE amounts to digest a certain amount of DNA is in the file of Lecture 1.

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PDF ikon 2014-09-08 - Lecture 1: The Basics (PDF)
Fájl 2014-09-08 - Lecture 1: The Basics (ODP)
PDF ikon 2014-09-12 - Lecture 2: Expression Systems (PDF)
Fájl 2014-09-12 - Lecture 2: Expression Systems (ODP)
PDF ikon 2014-09-19 - Lecture 3: Measuring Proteins (PDF)
Fájl 2014-09-19 - Lecture 3: Measuring Proteins (ODP)
PDF ikon 2014-09-24 - Lecture 4: Protein Tagging (PDF)
Fájl 2014-09-24 - Lecture 4: Protein Tagging (ODP)
PDF ikon 2014-10-17 - Lecture 5: "New cloning" methods (PDF)
Fájl 2014-10-17 - Lecture 5: "New cloning" methods (ODP)
Csomag ikon 2014-10-17 - Lecture 5: Further reading (ZIPped collection of PDF articles about the "new" "cloning" methods)
PDF ikon 2014-10-24 - Lecture 6: Mutagenesis and in-vitro evolution (PDF)
Fájl 2014-10-24 - Lecture 6: Mutagenesis and in-vitro evolution (ODP)
Csomag ikon 2014-10-24 - Lecture 6: Further reading (ZIPped collection of PDF articles about mutagenesis and in-vitro evolution methods)
PDF ikon 2014-10-31 - Lecture 7: Software for wetlab scientists (PDF)
Fájl 2014-10-31 - Lecture 7: Software for wetlab scientists (ODP)
PDF ikon 2014-11-07 - Lecture 8: Sequence sources and vector anatomy (PDF)
Fájl 2014-11-07 - Lecture 8: Sequence sources and vector anatomy (ODP)
Csomag ikon 2014-11-07 - Lecture 8: Further reading (ZIPped collection of articles about vector design/choice and high thruput cloning)
PDF ikon 2014-11-14 - Lecture 9: The Ins and Outs of Knock-Ins and Knock-Outs (PDF)
Fájl 2014-11-14 - Lecture 9: The Ins and Outs of Knock-Ins and Knock-Outs (ODP)
PDF ikon 2014-11-28 - Lecture 10: Recombinant Viruses (PDF)
Fájl 2014-11-28 - Lecture 10: Recombinant Viruses (ODP)