Kapa HiFi excels, Phusion works sort-of, Q5 and Platinum SuperFi disappoint
By jeltsch on Fri, 12/02/2016 - 21:33
My last NEBuilder assembly got stuck because I couldn't get two of the PCR reactions to work. So I tested alternatives to the Phusion DNA polymerase, which I have been using exclusively for cloning purposes during the last years...
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