GE Healthcare

Purification and Characterization of Recombinant Proteins

Size exclusion chromatogram (acetone performance test)

We are organizing (again) a practical hands-on protein purification course from December 4th to 20th. We maximally can accommodate 16 participants, which will form groups of 2 to 4 participants.

English

Unicorn 7 user separation requires manual intervention in a network user environment

Unicorn 7 user setup

Since we are operating our Äkta Avant in a multiuser environment, we need to separate the methods and results of the different users.

English

The logic of the Äkta Avant fraction collector

Äkta Avant inbuilt fraction collector

After we solved all the teething troubles with our Äkta Avant, we finally dare to let customers use it. Most of them find it difficult to understand the behaviour of the fraction collector...

English

HiLoad 26/60 Superdex pg gel filtration chromatography column performance

HiLoad 25/60 Superdex 200 pg

The most common protein purification technique that we use is gel filtration (also called size exclusion chromatography). In gel filtration, proteins are separated by their size (or more correctly by their "Stokes radius", which is largely determined by their size and shape). For gel filtration of large protein amounts, we bought GE Healthcare's HiLoad 26/60 Superdex 200 prep grade about 9 years ago. The performance of the column is outstanding. Being used to 30-cm-columns, we were amazed how well the column separates between e.g. dimeric and monomeric forms of our pet protein. However, the column lost dramatically its performance recently...

English

Review of GE Healthcare's Äkta Avant 25

Äkta Avant 25

About one year ago, we secured funding in an internal faculty call to replace the old Pharmacia Äkta Explorer 100 FPLC machine of the Protein Production and Purification core facility. The Äkta Explorer had been purchased in 1996(?) when the Swedish brand Pharmacia still existed. In 2014, the worse case scenerio happened and both monochromator and Xenon flash lamp broke almost simultaneously, which left us with a bill of almost 10000€. Hence we wanted to replace the machine with a contemporary model.

English

FPLC Protein purification course

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