January 1997 (Master's Thesis Seminar): Functional Analysis of VEGF-B and VEGF-C

Title slide: Functional Analysis of VEGF-B and VEGF-C

cell communication

classification of cytokines

crystal structure of PDGF-BB

amino acid sequence alignment of PDGF family members

vasculogenesis

angiogenesis

angiogenesis in the adult organism

VEGFs and VEGFRs

exon structure of VEGFs

receptor specificity within the VEGF family

aim, hypothesis &amp; approaches

baculovirus expression system

FastBac system

VEGF-C modifications to facilitate recombinant protein production &amp; purification

Production &amp; purification flow scheme for VEGF-C

Applications of recombinant VEGF-C

K14-hVEGF-C transgene expression vector map

K14-VEGF-C transgenic mouse with wt littermate

skin H&amp;E staining of K14-VEGF-C transgenic mouse

in-situ hybridisation of K14-VEGF-C transgenic mouse

IHC of K14-VEGF-C transgenic mouse

EM of K14-VEGF-C transgenic mouse

skin H&amp;E staining of K14-VEGF-B167 transgenic mouse

Conclusions

Acknowledgements