Practical Course: Purification and Characterization of Recombinant Proteins (DPBM-135)

Teaching material, results, etc. for the DPBM course “Purification and Characterization of Recombinant Proteins” ( https://courses.helsinki.fi/en/DPBM-135/120171139  ):

• FPLC (Fast Protein Liquid Chromatography)

Until I add the new data, please refer to this documentation: /en/FPLC-course .

Protocol (draft version)

• Protein Purification with the Äkta Avant (PDF)
• Protein Purification with the Äkta Avant (editable, Scribus file format)

Software

• Bio-Rad’s ChromLab software for FPLC control and analysis (you need to create an account to get to the download) 

Results

• Annotated, representative FPLC results of group 2 & 5 (more to come…)
• Scans of the Coomassie-stained SDS-PAGE gels

ITC (Isothermal titration calorimetry)

Some Very Brief Introduction

• The Working Principle of Isothermal Titration Calorimetry: https://www.youtube.com/watch/?v=o_IpWcWKNXI 
• Experimental Techniques Explained: Isothermal Titration Calorimetry: https://antisensescienceblog.wordpress.com/2013/11/15/experimental-techniques-explained-isothermal-titration-calorimetry-itc/ 
• A nice lecture series by Lars Wadsö (Lund University), that was unfortunately never completed: https://www.calmetrix.com/lectures-calorimetry 

Device Documentation

• PEAQ-ITC Getting Started Guide
• PEAQ-ITC User Manual (English)
• PEAQ-ITC Operating Instructions (English)
• PEAQ-ITC Analysis Software User Manual (English)

Documentation Videos

• Cleaning the instrument
• Loading the instrument

Software

• Setup MicroCal PEAQ-ITC Analysis Software
• Setup MicroCal PEAQ-ITC Control Software