Miscellaneous ramblings about science-related matters that matter to me (all blog posts that have been tagged with the keyword "science").

Kapa HiFi excels, Phusion works sort-of, Q5 and Platinum SuperFi disappoint

Comparison of PCR polymerases

My last NEBuilder assembly got stuck because I couldn't get two of the PCR reactions to work. So I tested alternatives to the Phusion DNA polymerase, which I have been using exclusively for cloning purposes during the last years...


Key project funding

Funding rates

This post is inspired by our recent success in the Key Project Funding call of the Academy of Finland. One of the main time killers for scientists is to apply for money to finance their research. Scientists spend more and more time preparing grant applications because of the growing competition for the shrinking resources.