Linux

I want to store all sequence traces of a sequencing project (using Staden) in one file. You can do that if you simply tar all the *.ztr files: tar -cvf ./test.tar *.ztr Then you have to specify in gap4 Options -> Configure menus -> Expert And then you need to specify Options -> Trace file location -> TAR=./test.tar

To make one sequence a reference sequence, just select its name from the gap4 Edit Contig window and right click and select as reference sequence. If the file is in embl format, the features will show up as Staden tags in the alignment.

KMail has always problems with multiple smtp servers. As a solution I installed sendmail. Then I just created a new outgoing mailserver and selected the sendmail option. No further configuration was necessary and it seems to work.