An impossible overlap-extension PCR
By jeltsch on Sat, 06/21/2025 - 15:59
A PhD student of mine asked me about plasmid maps for several DNA constructs that I had created some 20 years ago.
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The fact that everything looks different doesn't mean that all has changed...
SnapGene
Opening old plasmid map files
By jeltsch on Sat, 01/01/2022 - 21:39
For a new cloning project, we needed to access the plasmid maps of an old construct of mine (pSecTagN2, which was a precursor of pMosaic, aka pSecTagI) which I had composed from many different sources in 1999.
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Assembly of an OCAA collection Entry vector (pENTR221)
By jeltsch on Fri, 10/30/2020 - 20:44
This article is for people, who do molecular cloning. More specifically for people who need to deal with Gateway vectors. Let's assume you received a Gateway clone from somebody.
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