An impossible overlap-extension PCR
By jeltsch on Sat, 06/21/2025 - 15:59
A PhD student of mine asked me about plasmid maps for several DNA constructs that I had created some 20 years ago.
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The fact that everything looks different doesn't mean that all has changed...
mutagenesis
Low-budget PCR-based mutagenesis kit
By jeltsch on Thu, 11/26/2020 - 10:33
If you need to modify your DNA construct, one frequently used method is the PCR-based mutagenesis, where you incorporate the mutation into the middle of two complementary primers.
English