Practical Molecular Biology and Genetic Engineering

Collection of the presentation slides for the DPBM course Practical Molecular Biology and Genetic Engineering. There are files in (at least) two different formats for each lecture: PDF and ODP (Open Document Presentation). The ODP file is editable using LibreOffice software. If you want to open it with Microsoft Office, you need to convert it first using either LibreOffice or some online conversion tool (like cloudconvert). All material is available under the creative commons license. I might add improved versions of the lecture slides later based on participants' feedback. The slide about restriction enzymes (REs) and how to calculate the necessary RE amounts to digest a certain amount of DNA is in the file of Lecture 1.

English
PDF icon 2014-09-08 - Lecture 1: The Basics (PDF)
File 2014-09-08 - Lecture 1: The Basics (ODP)
PDF icon 2014-09-12 - Lecture 2: Expression Systems (PDF)
File 2014-09-12 - Lecture 2: Expression Systems (ODP)
PDF icon 2014-09-19 - Lecture 3: Measuring Proteins (PDF)
File 2014-09-19 - Lecture 3: Measuring Proteins (ODP)
PDF icon 2014-09-24 - Lecture 4: Protein Tagging (PDF)
File 2014-09-24 - Lecture 4: Protein Tagging (ODP)
PDF icon 2014-10-17 - Lecture 5: "New cloning" methods (PDF)
File 2014-10-17 - Lecture 5: "New cloning" methods (ODP)
Package icon 2014-10-17 - Lecture 5: Further reading (ZIPped collection of PDF articles about the "new" "cloning" methods)
PDF icon 2014-10-24 - Lecture 6: Mutagenesis and in-vitro evolution (PDF)
File 2014-10-24 - Lecture 6: Mutagenesis and in-vitro evolution (ODP)
Package icon 2014-10-24 - Lecture 6: Further reading (ZIPped collection of PDF articles about mutagenesis and in-vitro evolution methods)
PDF icon 2014-10-31 - Lecture 7: Software for wetlab scientists (PDF)
File 2014-10-31 - Lecture 7: Software for wetlab scientists (ODP)
PDF icon 2014-11-07 - Lecture 8: Sequence sources and vector anatomy (PDF)
File 2014-11-07 - Lecture 8: Sequence sources and vector anatomy (ODP)
Package icon 2014-11-07 - Lecture 8: Further reading (ZIPped collection of articles about vector design/choice and high thruput cloning)
PDF icon 2014-11-14 - Lecture 9: The Ins and Outs of Knock-Ins and Knock-Outs (PDF)
File 2014-11-14 - Lecture 9: The Ins and Outs of Knock-Ins and Knock-Outs (ODP)
PDF icon 2014-11-28 - Lecture 10: Recombinant Viruses (PDF)
File 2014-11-28 - Lecture 10: Recombinant Viruses (ODP)

Comments

Sara (not verified) - Mon, 09/08/2014 - 16:46

Another limitation with restriction enzymes is the amount of glycerol allowed in the reaction. Some enzymes like BamHI exhibit star activity at high glycerol concentrations. Star activity means that the enzyme cleaves at places where it is not supposed to cleave. Since restriction enzymes are stored as solutions containing 50% glycerol (to prevent freezing at -20°C), there is a maximum amount of enzymes one can add to a cleavage reaction. Thanks to Denis for pointing that out!