DNA

Uncertainty about CRISPR's future

Cas9 on a DNA helix

Some feared, that the patent decisions on the CRISPR technology this spring might lead to a monopolization of the technology. However, according to a last month's article in Nature Methods, it is not at all clear at this moment, whether CRISPR will hit a home run for the editing of the human genome.

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The Staden package on Ubuntu for bioinformatics dinosaurs

Installing Staden on Ubuntu 16.04

Mostly we use the SnapGene software when we check the sequences of our DNA constructs.

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Real biohacking

How much PCR product can you get?

PCR tube

How much PCR product does one get from a typical PCR reaction? 50-120 ng/µl seems to be a typical result, but it very much depends on your PCR conditions.

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SnapGene - Simply the best DNA manipulation software

Plasmid map of pBR322

Our lab has been using different software packages to plan, document and visualize DNA constructs.

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Staden problems

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