Miscellaneous ramblings about science-related matters that matter to me (all blog posts that have been tagged with the keyword "science").
When I started as a principal investigator at the University of Helsinki, I was advised to take the "Good leadership" course which was arranged by Personnel Training. Together with 15 other research group leaders, I learned between November 2013 and March 2014 from Sanna-Marja Heinimo and other experts i.a. the "skills for effectively developing, coaching, empowering and leading others to getting best results".
The most common protein purification technique that we use is gel filtration (also called size exclusion chromatography). In gel filtration, proteins are separated by their size (or more correctly by their "Stokes radius", which is largely determined by their size and shape). For gel filtration of large protein amounts, we bought GE Healthcare's HiLoad 26/60 Superdex 200 prep grade about 9 years ago. The performance of the column is outstanding. Being used to 30-cm-columns, we were amazed how well the column separates between e.g. dimeric and monomeric forms of our pet protein. However, the column lost dramatically its performance recently...